Sermorelin Testing

MW: 3357.88 g/molHalf-life: ~10–20 minutesStatus: Previously FDA-approved

Synthetic GHRH (1-29) analog. Short half-life.

Mechanism of action

GHRH(1-29), the minimum N-terminal fragment retaining full agonist activity at the GHRH receptor. Stimulates pulsatile GH release.

Research areas

  • Pediatric GH deficiency (historical FDA indication)
  • Age-related GH decline research
  • Body composition

What we test on Sermorelin

RP-HPLC with shallow gradient to resolve deamidation isomers; intact mass and MS/MS confirmation; endotoxin for injectables.

Standard GH Secretagogues Panel
  • RP-HPLC purity
  • ESI-MS identity
  • Amino acid analysis
  • Residual solvents (GC-MS)

Common impurities & failure modes

  • Asn8 deamidation

    Primary stability indicator; produces Asp/iso-Asp variants visible as 1–3 minute earlier-eluting peaks.

  • Met27 oxidation

    +16 Da shift.

  • Sequence truncations

    Standard SPPS failures, particularly the C-terminal Arg-Ser-Arg stretch.

Regulatory status

Previously FDA-approved (Geref, Geref Diagnostic) — withdrawn for commercial reasons, not safety.

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