PT-141 (Bremelanotide) Testing

MW: 1025.18 g/molHalf-life: ~2 hoursStatus: FDA-approved

Melanocortin-receptor agonist derived from Melanotan II. FDA-approved as Vyleesi.

Mechanism of action

Bremelanotide is a cyclic melanocortin receptor agonist (primarily MC4R and MC1R) derived from Melanotan II. Centrally mediated effects on sexual response — distinct from PDE5 inhibitors which act vascularly.

Sequence & structure

Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-OH

Cyclic via a lactam bridge between Asp and Lys side chains. Cyclization confirmation is essential — linear precursor is the most common impurity.

Research areas

  • Hypoactive sexual desire disorder (FDA-approved as Vyleesi)
  • Erectile function research
  • Melanocortin-pathway studies

What we test on PT-141 (Bremelanotide)

RP-HPLC purity, intact-mass LC-MS (to confirm cyclization), MS/MS sequence with stereochemistry confirmation, endotoxin.

Standard Sexual Health Panel
  • RP-HPLC purity
  • LC-MS (cyclization confirmation)
  • Disulfide bond mapping

Common impurities & failure modes

  • Linear (uncyclized) precursor

    +18 Da vs. main peak; primary identity QC parameter for any cyclic peptide.

  • Racemized D-Phe → L-Phe

    Eliminates receptor selectivity; chiral analysis required.

  • Trp oxidation

    +16 / +32 Da peaks.

  • Loss of N-terminal acetyl

    −42 Da shift.

Regulatory status

FDA-approved (Vyleesi 2019).

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